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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Cancer Res. 2021 Jun 11;81(15):4054–4065. doi: 10.1158/0008-5472.CAN-20-3792

Figure 4. TRA and 2DG has a synergistic effect to induce apoptosis in mouse or human PDAC cell lines.

Figure 4.

(A) Crystal violet staining of the cells treated with 25nM Tra, 1mM 2DG or combination for 4 days. (B) Quantification of crystal violet staining in B (n=3, Mean ± SD). (C-D) Representative Bliss score for combination of Tra and 2DG in iKras or IMR90 cell. (E) The synergistic effect of Tra and 2DG combination was analyzed using Bliss score in mouse (iKras and LSL-Kras), human PDAC line (HPAC and 8988T) and IMR90 cell line. (F) Quantification of crystal violet staining for the cell treated with SCH772984 (200nM)/GDC063 (100nM), 2DG (1mM) or combination (n=3, Mean ± SD). (G) The Bliss synergy score was calculated for the combination of Tra/2DG, gemcitabine/2DG and paclitaxel/2DG in iKras cell. (H) Apoptosis of the cell treated with 25nM Tra, 1mM 2DG or combination by annexin V/7-AAD staining (n=3, Mean ± SD).