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. 2021 Aug 4;12:4700. doi: 10.1038/s41467-021-25065-w

Fig. 7. NK cells contribute to bacterial killing in a HIF-1α-dependent manner.

Fig. 7

a FACS analysis of NK cell viability after 4 h of coculture of splenocytes purified from HIF-1α WT, HIF-1α KO, VHL WT and VHL KO animals with GAS in normoxia (20% O2) or hypoxia (2% O2) relative to NK cell viability without GAS coculture, pooled data of 2, (n = 6 mice). b FACS analysis of NK cell (NKp46+, NK1.1+) activation (IFNγ+ and CD107a+) in splenocytes from HIF-1α WT, HIF-1α KO, VHL WT and VHL KO animals after stimulation with heat-inactivated GAS (hi GAS) for 6 h under normoxia (20% O2) and hypoxia (2% O2) relative to culture without GAS, pool data of 2 experiments, (n = 6). c Killing of GAS by purified splenic NK cells HIF-1α WT, HIF-1α KO, VHL WT and VHL KO. NK cells were cocultured with GAS at a multiplicity of infection (MOI) equal to 1 under normoxic (20% O2) or hypoxic (2% O2) conditions for 4 h. CFUs are represented as fold change relative to the corresponding WT NK cells, pooled data of 3 experiments, (n = 11 for HIF-1α WT, n = 10 HIF-1α KO, n = 8 for VHL WT and n = 8 for VHL KO). Data are mean values ± SEM, statistical analysis: two-tailed Student’s t test.