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. 2021 Aug 4;12:4696. doi: 10.1038/s41467-021-24964-2

Fig. 2. Puf6 is required for 60S pre-ribosome export at LT.

Fig. 2

a Whole-cell extracts (WCE) from WT yeast cells grown at indicated temperatures were separated by SDS-PAGE and analyzed by Western blotting using a Puf6-specific antibody. Arc1 was used as a loading control. Protein levels were quantified by ImageJ software. The right panel depicts Puf6 protein levels normalized to the loading control Arc1. arb. units stand for arbitrary units. b Whole-cell extracts were prepared from WT yeast cells grown at 37 °C, and then shifted to 20 °C or vice versa for the indicated times were separated by SDS-PAGE and analyzed by Western blotting with a Puf6-specific antibody. Arc1 was used as a loading control. c puf6Δ cells expressing WT-Puf6 and human Pum3 were spotted in serial tenfold dilutions on selective minimal medium plates and grown at indicated temperatures for 3–5 days. d The indicated strains expressing uL18-GFP and uS5-GFP were grown to mid-log phase at 20 °C and at 30 °C. Localization of the reporters was visualized by fluorescence microscopy. bud20Δ and yrb2Δ cells expressing uL18-GFP and uS5-GFP, respectively, were used as positive controls. Scale bars = 5 µm. Source data are provided as a Source Data file.