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. 2021 Aug 4;7:201. doi: 10.1038/s41420-021-00591-0

Fig. 6. Protective effect of cycloheximide (CHX) on cell death induced by glucose withdrawn.

Fig. 6

A Cell viability measurement of Coq7 KO cells after 4 days of culture in galactose medium. B Viability measurement of wild-type or Coq7 KO cells after 2 days of culture in the sugar-free medium. Resazurin assay was used for determining cell viability with the results presented as relative to glucose-grown control cells. Error bars represent SEM (n = 4). CHX: 3 µM. ****p < 0.0001 (one-way ANOVA followed by Tukey’s multiple-comparison test). C Western blot of LC3 protein in wild-type and DKO MEFs after 2 days of indicated treatments. HCQ: hydroxychloroquine. Scale bars = 100 µm.