Fig. 11. Excitation and collection optics for nanoparticle on a film.

a Custom-designed objective for excitation and collection of nanoparticle on a film for SERS. p-ATP monomolecular layer was assembled on the surface of silver film. b AOI dependent SERS spectra of NOF decorated by p-ATP monomolecular layer. c and d Directional emission of SERS signal (690 cm⁻¹ and 2212 cm⁻¹) from nanoparticle on a film substrate. e Schematics of the ATRc-SHINERS optics⁹⁵. Directional emission of SERS signal in Otto ATR-Raman, SHINERS and ATRc-SHINERS configurations from f Mica and g Si substrates. h Directional emission of SERS signal in ATR-Raman, SHINERS and ATRc-SHINERS configurations from an Au substrate. The Raman-scattered wavelength is 700 nm, corresponding to the Raman shift at 1,500 cm⁻¹. i Incident angle and thickness of water space-dependent electrical field on the Au surface. Excitation wavelength is 633 nm. j Local field enhancement with different excitation optics⁵³