Figure 4.

X-ray imaging and histopathology analysis of the lungs of SARS-CoV-2 infected mice. (A) X-ray imaging and (B). Histopathology analysis of the mice groups that were negative control (uninfected and no vaccination), positive control (only infection), dose 10¹³ group, and dose 10¹⁴ group (vaccinated and infected). (C) Immunohistochemistry analysis of the lung tissues. Paraffin block was prepared from SARS-CoV-2 cell culture and lung tissues of the challenge assay mice groups. Sections of 4 microns made from paraffin block were studied immunohistochemically with SARS/SARS-CoV-2 coronavirus nucleocapsid monoclonal antibody (B46F) (ThermoFisher Scientific, US). Infected Vero cells were used as positive control; Human lung and placental tissue were used as negative controls (50 µm). NA not applied. (D) Immunohistochemistry analysis (H-score) of the lung tissues. *p < 0.05.