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. 2021 Aug 4;11:15835. doi: 10.1038/s41598-021-95180-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Viability of DU 145 and LNCaP cells after expousure to 25, 200 ns PEFs (a–d). Cells were permeabilized in suspension in a 1-mm gap electroporation cuvette. Viability of the cells as a function of electric field frequency. (a, b) The effect of PEFs was measured at two different electric field intensities. (c, d) Viability of cells after exposure to PEFs in medium with different Ca²⁺ concentration. Viability was assessed with MTT assay. Graphs are representative of at least 3 independent experiments. Data are mean ± SD (n = 3–5 independent experiments). (*) indicates statistically significant differences between the sample and control (a, b), or pair of samples (c, d) (ANOVA, p < 0.05).