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. 2021 Aug 4;11:15817. doi: 10.1038/s41598-021-95166-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic relationships of Entamoeba species based on 292 total positions in the 18S rDNA gene. Tree was inferred using an unrooted maximum likelihood method. The evolutionary distances were computed using the General Time-Reversible Model modeled using a gamma distribution (shape parameter = 0.5). Bootstrap proportions > 50% (over 1000 replicates) are shown next to the branch nodes. Accession numbers for the reference sequences are listed behind the Entamoeba species name. The scale bar represents 0.2 substitution per site.