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The representative melting curves for p53 DBD without or with ATO
Recombinant p53 DBD was mixed without or with ATO at a molar ratio of 1:20 in 20 mM HEPES (pH 7.5) containing 150 mM NaCl for 16–20 h. Melting curves were then measured in the DSF assay and the calculated Tm is shown.