Figure 5.
PAb1620 IP assay of p53 mutants upon ATO treatment
p53 mutants were expressed in transfected H1299 cells upon treatment with 1 μg/mL ATO. The input and immunoprecipitated p53 was probed. Relative band intensity was measured by ImageJ and normalized for each immunoblotting. Bars represent mean ± SD derived from three PAb1620 immunoprecipitation experiments. Representative images from one immunoprecipitation experiment are shown. Figure reprinted with permission from (Chen et al., 2021)