Figure 6.

PAb1620 IP assay of p53-R175H upon ATO treatment by using NP40 buffer at different temperature

p53-R175H were expressed in transfected H1299 cells upon treatment with 1 μg/mL ATO. The input and immunoprecipitated p53 was probed. The beads were washed with either 20°C–25°C or 4°C NP40 buffer (step 21). Relative band intensity was measured by ImageJ and normalized for each immunoblotting. Bars represent mean ± SD derived from three PAb1620 immunoprecipitation experiments. Representative images from one immunoprecipitation experiment are shown. Figure reprinted with permission from (Chen et al., 2021)