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Luciferase reporter assay of the indicated p53 mutants on the target BBC3 (PUMA)
p53 mutants were expressed in H1299 cells upon treatment with 1 μg/mL ATO for 24 h. The fold-change of RLU upon ATO treatment is indicated. An optimized plasmid ratio was used. Bars represent mean ± SD (n = 3) and p-values are shown.
