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. 2021 Jul 27;2(3):100688. doi: 10.1016/j.xpro.2021.100688

Figure 9.

Figure 9

Immunostaining results of three cancer cell lines using p53 antibodies DO1 or PAb1620

The indicated cancer lines A549 (A), SK-MEL37 (B), and A431 (C) were pretreated with DMSO or 10 μM Nutlin for 24 h, followed by immunofluorescence staining using p53 antibodies DO1 or PAb1620, respectively. Scale bar = 50 μm .The right graph (mean ± SD) shows percentage of staining-positive cells. Three scopes (> 50 cells in each scope) for each sample were randomly picked for cell counting. The detail procedure of the experiment can be found in (Chen et al., 2021).