HEPES buffer for DSF assay

Reagent	Final concentration	Volume (mL)
4 M NaCl	150 mM	18.75
2 M HEPES pH 7.5	20 mM	5
ddH2O	N/A	476.25
Total	N/A	500

Keep the HEPES buffer at 2°C–8°C for up to 6 months.