Reagent | Final concentration | Volume (mL) |
---|---|---|
1 M Tris-HCl pH 8.0 | 50 mM | 25 |
4 M NaCl | 150 mM | 18.75 |
0.5 M EDTA | 1 mM | 1 |
NP40 | 1% (v/v) | 5 |
ddH2O | N/A | 450.25 |
Total | N/A | 500 |
Keep the NP40 buffer at 2°C–8°C for up to 6 months.
Reagent | Final concentration | Volume (mL) |
---|---|---|
1 M Tris-HCl pH 8.0 | 50 mM | 25 |
4 M NaCl | 150 mM | 18.75 |
0.5 M EDTA | 1 mM | 1 |
NP40 | 1% (v/v) | 5 |
ddH2O | N/A | 450.25 |
Total | N/A | 500 |
Keep the NP40 buffer at 2°C–8°C for up to 6 months.