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. 2021 Jul 27;2(3):100688. doi: 10.1016/j.xpro.2021.100688

LB medium

Reagent Final concentration Volume (mL)
Tryptone 10 g/L N/A
Yeast extract 5 g/L N/A
NaCl 10 g/L N/A
ddH2O N/A 1000
Total N/A 1000

Sterilize in autoclave at 121°C for 15 min. Store at 2°C–8°C for 1–3 months.