Lysis buffer A
Reagent | Final concentration | Volume (mL) |
---|---|---|
1 M Tris-HCl pH 7.0 | 50 mM | 25 |
4 M NaCl | 50 mM | 6.25 |
1 M DTT | 10 mM | 5 |
ddH2O | N/A | 463.75 |
Total | N/A | 500 |
Keep the lysis buffer A at 2°C–8°C for up to 6 months.
Lysis buffer A
Reagent | Final concentration | Volume (mL) |
---|---|---|
1 M Tris-HCl pH 7.0 | 50 mM | 25 |
4 M NaCl | 50 mM | 6.25 |
1 M DTT | 10 mM | 5 |
ddH2O | N/A | 463.75 |
Total | N/A | 500 |
Keep the lysis buffer A at 2°C–8°C for up to 6 months.