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Preparation of Standards for RT-qPCR
9 μL cDNA solution in total is mixed with 36 μL nuclease-free water (designated as Standard 1296). 6 μL is aliquoted from Standard 1296 and mixed with 30 μL nuclease-free water (designated as Standard 216). This is done serially to prepare Standard 36, 6, 1. Nuclease-free water is designated as Standard 0 and serves as blank control. NF-H2O, nuclease-free water.
