Recipe for qPCR premix
| Reagents | Final concentration | Amount |
|---|---|---|
| Nuclease-free water | n/a | 160 μL |
| Forward primer (10 μM) | 0.625 μM | 40 μL |
| Reverse primer (10 μM) | 0.625 μM | 40 μL |
| SYBR master mix (−20°C for 12 months) | n/a | 400 μL |
| Total | n/a | 640 μL |
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