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. 2021 Jul 22;12:663793. doi: 10.3389/fneur.2021.663793

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Copyright © 2021 Lin, Elbezanti, Schirling, Ahmed, Van Duyne, Cocklin and Klase.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of alprazolam ex vivo on RUNX1 responsive genes. Peripheral blood mononuclear cells (PBMC) from three HIV-1-1 positive individuals were treated with 10μM Alprazolam or Ro5-3335 for 48 h. RNA was harvested using Trizol and cDNA was synthesized by RT reaction. qPCR was performed for five known RUNX responsive genes: (A) APOBEC3C, (B) APOBEC3G, (C) Tbet, (D) IL-7R and (E) IL-2. Expression levels are plotted as GAPDH normalized expression with the levels in the control for each individual. Dots represent each individual data point and graphs show the average. ANOVA was used to determine statistical differences. *p < 0.05.