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. 2021 Jul 22;12:663793. doi: 10.3389/fneur.2021.663793

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Copyright © 2021 Lin, Elbezanti, Schirling, Ahmed, Van Duyne, Cocklin and Klase.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of Activated STAT5 was increased when TZMBL cells were treated with Alprazolam. TZMBl (A, B), HEK293T (C) and U87MG (D) cells were treated with Benzodiazepines: 50 μM RO5-3335, 10μM Alprazolam, or 10μM Clonazepam for 48 h. Cells were lysed and whole cell lysate was collected and loaded onto an SDS-PAGE Gel (A) Phospho-Tag SDS-PAGE was performed to evaluate the dose effect of alprazolam on STAT5 phosphorylation. From left to right, TZMBL cells were treated with DMSO, 0.1μM, 1μM and 10μM of Alprazolam. (B–D) Western Blot was performed to evaluate the effect of Benzodiazepines on STAT5 expression and phosphorylation (E) Densitometry was performed and the ratio of activated STAT5 over global STAT5 was graphed.