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Alprazolam activates a STAT5 sensitive reporter. HEK-Blue-IL2 cells were treated with indicated doses of IL-2, alprazolam or Ro5-3335 and incubated for 48 h at 37C 5% CO2. (A) Extracellular alkaline phosphatase activity was then measured by colorimetric assay. Results show the average absorbance of three replicates. **P ≤ 0.01, ****P ≤ 0.0001, ns = not significant (B) Western blot was performed to evaluate the effect of Benzodiazepines on STAT5 expression and phosphorylation.
