Figure 3.

LATS1 inhibits the expression of YAP and TAZ of the Hippo-YAP signaling pathway

(A) Protein band of LATS1 in hDPSCs measured by western blot analysis. (B) Protein level of LATS1 in hDPSCs measured by western blot analysis. (C) Protein bands of YAP and TAZ in hDPSCs measured by western blot analysis. (D) Protein levels of YAP and TAZ in hDPSCs measured by western blot analysis. (E) Expression of odontoblast differentiation-related genes (ALP, DSPP, OCN, and DMP1) in cells determined by quantitative real-time PCR. (F) ALP staining detection (×100). (G) ALP activity determination. (H) The formation of mineralized nodules examined by alizarin red staining (×200). (I) Relative mineralization of panel (H) graph. (J) Cell viability assessed by CCK-8 assay. (K) Cell migration evaluated by Transwell assay (×200). Each experiment was repeated 3 times independently. ∗p < 0.05, compared with the oe-NC group (hDPSCs transfected with oe-NC). #p < 0.05, compared with the si-NC group (hDPSCs transfected with si-NC).