Figure 4.

lncRNA H19 induces H3K27me3 and inhibits the expression of LATS1 by recruiting EZH2

(A) mRNA expression of LATS1 determined by quantitative real-time PCR after H19 was upregulated or silenced. (B) Western blots of LATS1 in hDPSCs. (C) Protein level of LATS1 in hDPSCs measured by western blot analysis. (D) Nuclear and cytoplasmic expression of lncRNA H19 in hDPSCs detected by quantitative real-time PCR. (E) Binding between lncRNA H19 and EZH2 assessed by RNA pull-down assay. (F) The enrichment of EZH2 by lncRNA H19 relative to IgG assessed by RIP assays. (G) Western blots of EZH2 in in hDPSCs. (H) Protein level of EZH2 in cells assessed by western blot analysis. (I) mRNA expression of LATS1 after upregulation or silencing of EZH2 determined by quantitative real-time PCR. (J) Western blots of LATS1 in hDPSCs after upregulation or silencing of EZH2 measured by western blot analysis. (K) Protein level of LATS1 in hDPSCs after upregulation or silencing of EZH2 measured by western blot analysis. (L) The enrichment of H3K27me3 on the LATS1 promoter after upregulation or silencing of EZH2 examined by ChIP assay. (M) The amount of H3K27me3 on the LATS1 promoter after upregulation or the silencing of lncRNA H19 detected by ChIP. Each experiment was repeated 3 times independently. ∗p < 0.05, compared with the oe-NC group (hDPSCs transfected with oe-NC). &p < 0.05, compared with the sh-NC group (hDPSCs transfected with sh-NC).