Figure 5.

lncRNA H19 induces the differentiation, proliferation, and migration capabilities of hDPSCs through the regulation of LATS1

(A) Western blots of YAP and TAZ in hDPSCs determined by western blot analysis. (B) Protein levels of YAP and TAZ in hDPSCs determined by western blot analysis. (C) mRNA expression of ALP, DSPP, OCN, and DMP1 in hDPSCs detected by quantitative real-time PCR. (D) ALP activity determination. (E) The formation of mineralized nodules examined by alizarin red staining. (F) Viability of hDPSCs assessed by CCK-8 assay. (G) Migration of hDPSCs evaluated by Transwell assay. Each experiment was repeated 3 times independently. ∗p < 0.05, compared with the oe-NC group (hDPSCs transfected with oe-NC). &p < 0.05, compared with the oe-H19 group (hDPSCs transfected with oe-H19).