Figure 7.

Classifications of OCRGs differentially expressed in endothelial cells treated with pro-atherogenic DAMPs such as oxLDL, LPS, oxPAPC, and IFNs treated. (A) When the overlapped genes in all upregulated OCRGs and in all downregulated OCRGs were removed, 33 genes are upregulated and 36 genes are downregulated exclusively in inflammatory factor-treated endothelial cells. (B) Donut chart shows the ratio of classification of upregulated OCRGs (left) and downregulated OCRGs (right) in pro-atherogenic DAMP-treated endothelial cells. There are 10 classifications in 33 upregulated and 36 downregulated OCRGs, respectively. There are no MT fission and fusion, MT translocation, MT biogenesis, MT contact site, autophagosome–lysosome fusion, and endosome–GC OCRGs in upregulated genes and not MT fusion, sarcoplasmic reticulum–MT, autophagosome–endosome/lysosome fusion, MT biogenesis, MT contact site, and endosome–GC OCRGs in downregulated genes. (C) There are 20 significant GO enrichment results in 33 upregulated OCRGs of pro-atherogenic DAMP-treated endothelial cells. Top 3 GO enrichments of OCRGs are membrane trafficking, autophagy, and mitochondrial fission. Wnt signaling pathway is upregulated, and this change suggests inflammatory factors may be via Wnt signaling pathway, promoting inflammation induced by OCRGs. (D) There are 14 significant GO enrichment results in 36 downregulated OCRGs in inflammatory factor-treated endothelial cells. Calcium ion transmembrane transporter activity signaling is downregulated, and this change suggests that inflammatory factors can change calcium ion transmembrane transporter activity via OCRGs in endothelial cells. Enrichment analysis was performed by using Metascape software (http://metascape.org/gp/index.html#/main/step1; PMID: 30944313).