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. 2021 Jul 22;8:687079. doi: 10.3389/fvets.2021.687079

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Copyright © 2021 Guo, Wang, Pan, Shi, Li, Guo and Xing.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The phylogenetic trees based on the whole genomes of SADS-CoVs (A) or the full-length spike glycoprotein ORF (B), ORF1a and ORF1b (C), or E protein ORF (D), M protein ORF (E), or N protein ORF (F). Multiple-sequence alignments were performed using Clustal Omega server and then phylogenetic trees were constructed using neighbor-joining method in the MEGA-X software (16, 17). The numbers at each branch represent bootstrap values >50% of 1,000 replicates. Scale bars indicate the number of inferred substitutions per site. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. Evolutionary distances were computed using the Maximum Composite Likelihood method (17).