FIGURE 5.

The role of rhKGF-2 SILI rat model. The SILI rat model was established by inhalation of smoke. Then, rhKGF-2 (10 ng/kg body weight), GSK2126458 (300 μg/kg body weight) and AS1842856 (10 μg/kg body weight) were used for treating the SILI rats, the same volume of saline was administered on the rat in the sham group. (A–C). The arterial blood of rats was extracted to detect the values of PaO₂, PaCO₂, PaO₂/FiO₂ to analyze the pulmonary function. The Y-axis of panel A and B represents the partial pressure of O₂ and CO₂ (mmHg). The Y-axis of panel C represents the Oxygenation index (the ratio of arterial partial pressure of oxygen to inhaled oxygen concentration) (mmHg). (D). The wet weight (W) and dry weight of the rat lung were measured, and the W/D ratio was calculated to evaluate the pulmonary edema. (E). HE staining was performed to observe the pathological changes of rat lung in each group. (F). The pathological scores of rat lung in each group were counted. (G). TUNEL assay was implemented to analyze apoptosis in the lung tissues. (H). The number of TUNEL-positive cells in an area of 0.1 mm² was calculated. Scale bar = 50 μm. Data are expressed as mean ± SD. All experiments were repeated for three times. Data difference was analyzed via ANOVA, and Student's t test. NSP>0.05, *p<0.05, **p<0.01, ***p<0.001. Five rats were involved in each group (N = 5).