FIGURE 7.

rhKGF-2 has a role in regulating the activation of PI3K/Akt pathway, Nrf2/HO-1/NQO1 pathway and FoxO1-NLRP3-ASC-Caspase1 inflammasome in vivo. The SILI rat model was established by inhalation of smoke. Then, rhKGF-2 (10 ng/kg body weight), GSK2126458 (300 μg/kg body weight) and AS1842856 (10 μg/kg body weight) were used for were used for treating the SILI rats, the same volume of saline was administered on the rat in the sham group. Left lung tissues of rats in each group were collected for detection. A1-A2: WB was carried out to determine the expression of PI3K/Akt and FoxO1 in the lung tissues. B1-B2. The protein levels of Nrf2, HO-1 and NQO1 in the lung tissues. or nuclear were detected by WB. Nuclear Nrf2 was calculated using Histone H3 as internal control. C1-C2. WB was conducted to detect NLRP3-ASC-Caspase1 in the lung tissues. The relative protein expression (Y-axis) was calculated as fold change of sham group. Data are expressed as mean ± SD. All experiments were repeated for three times. Data difference was analyzed via ANOVA, and Student's t test. NSP>0.05, *p<0.05, **p<0.01, ***p<0.001. Five rats were involved in each group (N = 5).