Fig. 4.

Synthetic RNA of SARS-CoV-2 D614G detected by dsmCRISPR. (A) Fluorescence signals of D614 (6 × 10⁴ cps) and 10-fold gradually diluted RNA templates of G614 (from 6 × 10⁴ cps to 6 × 10⁰ cps) detected by dsmCRISPR (n=3). NTS, non-target sequence. (B) The presented images of visual detection in (A) are shown. (C) After amplification, the products were analyzed by electrophoresis in 2% agarose gel. M represents 100 bp DNA ladder. (D) SARS-CoV-2 RNA fragments containing RNA G614 or D614 were mixed with or without total RNAs of HEK293T, then detected by dsmCRISPR NTS, non-target sequence. (E) The presented images of visual detection in (D) are shown. (F) After amplification, the products were analyzed by electrophoresis in 2% agarose gel. M represents 100 bp DNA ladder.