FIGURE 2.

Construction and identification of CD147 EC‐KO mice. (A) Strategies for generating endothelial cell‐specific CD147 knockout mice (EC‐KO) and WT littermates. (B) Representative PCR results of genotype identification. From top to bottom, the bands are Cre‐KI (411 bp), Cre‐WT (268 bp), loxp‐CD147 (405 bp) and wild‐type CD147 (371 bp). (C) Mouse brain slices were costained for CD31 (red), CD147 (green) and nuclei (blue) to verify CD147 knockout efficiency. Scale bars: 50 μm. (D) Diagram of the isolation of primary cortex ECs for further experiments (see the Methods). Validation of isolated cells via (E) immunofluorescence. Scale bars: 100 μm. (F) RT‐qPCR and (G) WB. Data are presented as the mean ± SEM of n = 3 independent experiments. ****p < 0.0001