FIGURE 8.

Zinc can reduce the inflammation of damaged parts by inhibiting ferroptosis. (A) The expressions of TNF‐α, IL‐6, IL‐1β, and ICAM‐1 were evaluated by Western blotting in VSC4.1 in each group (n = 6). (B) Quantification of TNF‐α, IL‐6, IL‐1β, and ICAM‐1 expressions (n = 6, all the data are expressed as means ± SD, two‐way ANOVA followed by Tukey's post hoc test was applied). (C–F) Immunofluorescence staining was used to detect the level of TNF‐α, IL‐6, IL‐1β, and ICAM‐1 from each group (n = 6, scale bar =50 µm). (G) Statistical analysis of immunofluorescence staining for positive expression of TNF‐α, IL‐6, IL‐1β, and ICAM‐1 in VSC4.1 from each group (n = 6, all the data are expressed as means ± SD, two‐way ANOVA followed by Tukey's post hoc test was applied). * means p < 0.05; **means p < 0.01; and *** means p < 0.001