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. 2021 Jul 22;12:675322. doi: 10.3389/fphys.2021.675322

FIGURE 1.

FIGURE 1

High dysferlin expression in mouse and human atherosclerotic lesions. (A) Dysferlin Ab and control detection in representative ApoE-null mouse aortic sections (scale bar = 40 μm) and human coronary lesions (scale bar = 600 μm and scale bar = 50 μm) using goat anti-dysferlin antibodies or IgG with hematoxylin counterstain. (B) Tissue specific detection and knockout efficiency of Dysferlin in mouse lysates (skeletal muscle, liver, thoracic aorta, and small intestine) via western blot using the mouse anti-dysferlin; NCL-Hamlet 1 antibody in wild type (WT) and Dysf (KO) mice. Quantification of Dysf Ab is shown relative to ponceau staining, and expressed as a percentage of the WT:skeletal muscle signal. (C) Genotyping PCR using ear clip DNA biopsies of wild type (WT), heterozygous mutant (HET), and homozygous mutant Dysf (KO) mice, as well as a no template control (NTC).