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. 2021 Jul 1;22(8):e52835. doi: 10.15252/embr.202152835

Figure EV4. Depletion of MHCIIlow and MHCIIhi ATM subsets has no effect on vasculature integrity.

Figure EV4

  1. Representative flow cytometry analysis (on the left) shows the staining profile of CD45 and CD31, and the chart (on the right) shows the percentage of CD31+CD45− fractions in the eWAT (ND 16 weeks) of five male control and five male CD169‐DTR (DT for 7 days) mice; data were expressed as mean ± SD. Statistical significance was determined using an unpaired Student’s t‐test. ns: no significant difference.
  2. Left: Representative Evans blue‐stained eWAT pictures obtained from WT or CD169‐DTR male mice (ND 16 weeks, DT for 7 days). Right: The chart shows the normalized (absorption values were normalized to tissue weight in grams) mean optical density ± SD (n = 4 mice per group). Statistical significance was determined using an unpaired Student’s t‐test. ns: no significant difference.
  3. Representative flow cytometry analysis (on the left) shows the staining profile of CD45 (x‐axis) and CD31 (y‐axis), and the chart (on the right) shows the percentage of CD31+ CD45− fractions in the eWAT (HFD 16 weeks) of 4 control and 4 anti‐CSF1R antibody‐treated mice; data were expressed as mean ± SD. Statistical significance was determined using an unpaired Student’s t‐test. ns: no significant difference.
  4. Left: Representative Evans blue‐stained eWAT pictures from WT or anti‐CSF1R antibody‐treated mice (HFD 16 weeks, after 12 days’ antibody treatment). Right: The chart shows the normalized (absorption values were normalized to tissue weight in grams) mean optical density ± SD (n = 4 mice per group). Statistical significance was determined using an unpaired Student’s t‐test. ns: no significant difference.
  5. Elastin staining showing similar ECM distribution in anti‐CSF1R antibody‐treated (HFD 16 weeks) mice compared with the ECM distribution in the control (DAPI, blue; elastin, red; and CD31, green). The final image shows the other images merged together (n = 3–5 mice per group). Scale bar: 50 μm.
  6. Representative picrosirius red staining showing comparable collagen expression in anti‐CSF1R antibody‐treated eWAT (HFD 16 weeks) compared with collagen expression in WT control mice (n = 3–5 mice per group). Scale bar: 100 μm.