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. 2021 Jun 8;22(8):e52032. doi: 10.15252/embr.202052032

Figure EV4. STAT1‐response facilitates immune recognition of ArCK cells.

Figure EV4

  1. Significantly differentially expressed STAT1 target genes in ArCK cells compared with euploid control cells were identified by ingenuity pathway analysis based on RNA sequencing data. (Log2 fold change, p‐value ≤ 0.05).
  2. Measurement of STAT1 mRNA levels in ArCK cells normalized to euploid control cells. n = 3 biological replicates; mean ± SEM. **P = 0.0025; unpaired t‐test.
  3. Measurement of STAT1 protein levels in STAT1 KO single cell clones generated in RPE1‐hTERT cells.
  4. GSEA enrichment plot for interferon alpha and interferon gamma hallmarks in ArCK cells generated in either control or STAT1 KO RPE1‐hTERT cells. Two single cell cloned control cell lines and three single cell cloned STAT1 KO cell lines (shown in C) were used in the RNA‐seq analysis.
  5. ArCK cells lacking STAT1 were generated as described in the Method section. NK cell‐mediated cell death in STAT1 ArCK cells was compared with control cells which harbored an empty vector. n = 4 biological replicates; mean ± SEM. ArCK‐c1 vs. ArCK STAT1‐c1, P = 0.7, n.s.; ArCK‐c1 vs. ArCK STAT1‐c2, P = 0.05, n.s.; ArCK‐c1 vs. ArCK STAT1‐c3, P = 0.14, n.s.; KS test.