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. 2021 Jul 22;11:699889. doi: 10.3389/fonc.2021.699889

Figure 4.

Figure 4

PTK7 regulates EGFR-PI3K-Akt pathway in breast cancer. (A) PTK7 co-expression analysis in invasive breast carcinoma using breast cancer datasets including 1,904 patients with Agilent microarray data (http://www.cbioportal.org/) and 374 PTK7 positively- (Spearman’s correlation > 0.3, P < 0.01) and 289 PTK7 negative- (Spearman’s correlation < -0.3, P < 0.01) correlated genes were selected and used as candidate genes in the following analysis. (B) PTK7 positively-correlated genes were analyzed using Kyoto Encyclopedia of Genes and Genomes (KEGG) by DAVID: Functional Annotation Tools (https://david.ncifcrf.gov/tools.jsp). PTK7 positively correlated genes enriched in PI3K-Akt signaling (hsa04151) and Regulation of actin cytoskeleton (hsa04810) pathways are listed in the frames, respectively. (C) PTK7 and EGFR pair-wise gene correlation in breast invasive carcinoma were analyzed using GEPIA Correlation Analysis tools (http://gepia.cancer-pku.cn/detail.php?clicktag=correlation). (D, E) MDA-MB-468 (D) and MDA-MB-231 (E) cells were transduced with a non-targeting control shRNA (shNC) or two different PTK7-specific shRNAs (shPTK71 and shPTK7#2). Cells were stimulated with EGF (500 ng/ml) for 0, 20 or 40 minutes and phospho-EGFR and phosphor-Akt levels were evaluated using immunoblotting assay.