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. 2021 Jul 20;22(8):e52071. doi: 10.15252/embr.202052071

Figure EV4. Exophers contain muscle‐produced vitellogenin.

Figure EV4

  1. Muscular exophers formation does not depend on the apoptotic engulfment pathway proteins CED‐1 and CED‐6. n = 114–120; N = 3.
  2. CED‐1 depletion leads to a small increase in VIT‐2::GFP in embryos. n = 25 and 26; N = 2.
  3. Images show the formation of an exopher filled with the proteasome and vitellogenin. Images were captured before and after RPN‐5::RFP photobleaching, confirming that the high signal from endogenous VIT‐2::GFP in forming exophers is not an imaging artifact. The white circle marks the bleached area using a 555‐nm laser and the position of the developing exopher. Dashed lines mark different tissue borders: m—muscle, i—intestine, g—gonad. Scale bar is 10 µm.

Data information: Data are shown as mean ± SEM; n represents the number of worms; N represents the number of experimental repeats that were combined into a single value; ns—not significant ** P < 0.01; (A, B) Mann–Whitney test.