Reagent or Resource	Source	Identifier
Bacterial and Virus Strains
Escherichia coli RNAi feeding strain	Caenorhabditis Genetics Center	HT115(DE3)
E. coli feeding strain	Caenorhabditis Genetics Center	OP50
Ahringer RNAi library	Source BioScience	C. elegans RNAi Collection (Ahringer)
Chemicals, Peptides, and Recombinant Proteins
Bacto Agar	BioShop	AGR001.1
Bacto Peptone	BioShop	PEP403.1
Sodium Chloride	Chempur	117941206
Streptomycin sulfate salt	Sigma‐Aldrich	S6501
Nystatin suspension 10,000 unit/mL	Sigma‐Aldrich	N1638
Carbenicillin disodium salt	Roth	6344.2
Magnesium Sulfate heptahydrate	Sigma‐Aldrich	M5921
Potassium dihydrogen phosphate	Roth	3904.1
di‐potassium hydrogen phosphate	Roth	P749.1
Calcium Chloride (dihydrate)	BioShop	CCL444.500
Cholesterol	BioShop	CHL500.5
Sodium azide	Sigma‐Aldrich	S2002
Tetracycline HCl	BioShop	TET701
IPTG	BioShop	IPT001
Methylviologen dichloride hydrate	Sigma‐Aldrich	36541
Polystyrene microspheres	Polysciences Europe GmbH	08691‐10
Tetramisole hydrochloride	Sigma‐Aldrich	L9756
Paraformaldehyde	Sigma‐Aldrich	158127
Hepes	Roth	HN77.3
Glutaraldehyde	Sigma‐Aldrich	340855
Ruthenium red	Sigma‐Aldrich	R2751
Gelatin	BioShop	GEL771
Osmium tetroxide	Polysciences Europe GmbH	0972B‐5
Potassium ferrocyanide	Sigma‐Aldrich	P3289
Thiocarbohydrazide	Sigma‐Aldrich	88535
Hydrogen peroxide solution	Sigma‐Aldrich	H1009
5‐Fluoro‐2'‐deoxyuridine	Sigma‐Aldrich	F0503
Sodium hypochlorite solution 14%	VWR	27900
Experimental Models: Organisms/Strains
C. elegans: wacIs6[myo‐3p::pas‐7::GGGGS Linker‐wrmScarlet::unc‐54 3′UTR, unc‐119(+)], wacIs14[myo‐3p::tomm‐20_1–50aa::attB5::mGFP::unc‐54‐3′UTR, unc‐119(+)]	This paper	Strain: ACH91
C. elegans: wacIs1[myo‐3p::rpn‐5 CAI = 0.97::GGGGS Linker‐wrmScarlet::unc‐54 3′UTR, unc‐119(+)], wacIs14[myo‐3 promoter::tomm‐20_1–50aa::attB5::mGFP::unc‐54‐3′UTR, unc‐119(+)]	This paper	Strain: ACH93
C. elegans: wacIs1[myo‐3p::rpn‐5 CAI = 0.97::GGGGS Linker‐wrmScarlet::unc‐54 3′UTR, unc‐119(+)], vit‐2(crg9070[vit‐2::gfp]) X	This paper	Strain: ACH199
C. elegans: rrf‐3(b26) II; fem‐1(hc17) IV; uthEx633 [myo‐3p::GFP]	Caenorhabditis Genetics Center	Strain: AGD885
C. elegans: wacIs1[myo‐3 promoter::rpn‐5 CAI = 0.97::GGGGS Linker‐wrmScarlet::unc‐54 3′UTR, unc‐119(+)], wacIs14[myo‐3 promoter::tomm‐20_1–50aa::attB5::mGFP::unc‐54‐3′UTR, unc‐119(+)], wwaEx2[unc‐122 promoter::GFP]	This paper	TUR5
C. elegans: him‐5(e1490)V	Caenorhabditis Genetics Center	Strain CB4088
C. elegans: zcIs14 [myo‐3::GFP(mit)]	Caenorhabditis Genetics Center	Strain SJ4103
C. elegans: Bristol (N2) strain as wild‐type (WT)	Caenorhabditis Genetics Center	Strain: N2
Recombinant DNA
Plasmid: pCG150 (destination vector)	Addgene	#17247
Plasmid: pMT26 (modified pCG150)	This paper	N/A
Plasmid: pSEM89 (wrmScarlet template)	Boulin Lab	El Mouridi et al (2017)
Plasmid: L1_Tomm20_1–55aa_R5 (tomm‐20 1–55aa template)	This paper/ synthesized by Invitrogen GeneArt Gene Synthesis	N/A
Plasmid: L1_rpn‐5_CAI097_R5 (rpn‐5 CAI = 0.97 template)	This paper/ synthesized by Invitrogen GeneArt Gene Synthesis	N/A
Plasmid: L1_pas‐7_R5 (pas‐7 template)s	This paper/ synthesized by Invitrogen GeneArt Gene Synthesis	N/A
Plasmid: mGFP (mGFP template)	Bringmann Lab	N/A
Plasmid: pMT24 (pmyo‐3::pas‐7::wrmScarlet::unc‐54‐3′UTR in pCG150)	This paper	N/A
Plasmid: pMT28 (pmyo‐3::tomm‐20 1–55aa::mGFP::unc‐54‐3′UTR in pCG150)	This paper	N/A
Plasmid: pMT29 (pmyo‐3::rpn‐5 CAI = 0.97::wrmScarlet::unc‐54‐3′UTR in PCG150)	This paper	N/A
Oligonucleotides
For the list of oligonucleotides see Appendix Table S3	N/A	N/A
Software and Algorithms
GraphPad Prism 8	GraphPad Software, Inc.	N/A
ZEN	Zeiss	N/A
Office 365	Microsoft	N/A
ImageJ	Wayne Rasband (NIH)	N/A
WormLab	MBF Bioscience	N/A
Leica Las X	Leica	N/A
Adobe Illustrator 2020	Adobe	N/A
eC‐CLEM	Open Source	N/A