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. 2021 Jul;9(13):1087. doi: 10.21037/atm-21-2829

Figure 2.

Figure 2

Small molecules efficiently induce hUC-MSC to differentiate to DE. (A) Morphological changes during stage I differentiation. Scale bar =100 µM. (B) qRT-PCR analysis of the mRNA level expressed during DE differentiation stage. (C) Immunofluorescence of DE marker at day 2 (small molecule-induced DE cells) and day 3 (growth factor-induced DE cells). Scale bar =100 µM. (D) Percentage of FOXA2+SOX17+ in the immunofluorescence of DE cells. Undifferentiated human MSCs used as control. All data are presented as the mean of at least three independent experiments. *P value <0.05; ****P value <0.005. qRT-PCR, quantitative real-time PCR; SM, small molecule; GF, growth factor; MSC, mesenchymal stem cell; DE, definitive endoderm.