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. 2021 Jul;9(13):1087. doi: 10.21037/atm-21-2829

Figure 3.

Figure 3

Small molecules efficiently induce DE to differentiate to hepatic progenitors. (A) Morphological changes during hepatic progenitor differentiation. Scale bar =100 µM. (B) qRT-PCR analysis of the mRNA level of hepatic progenitor specific markers expressed in small molecule or growth-factor groups. (C) Immunofluorescent of AFP and HNF4α protein level expression at day 5 (small molecule-induced hepatic progenitor) and day 10 (growth-factor hepatic progenitor). Scale bar =100 µM. (D) Percentage of AFP+ HNF4α+ in immunofluorescence of hepatic progenitors. Undifferentiated human MSCs used as control. All data presented as the mean of at least three independent experiments. *P value <0.05. qRT-PCR, quantitative real-time PCR; SM, small molecule; GF, growth factor; MSC, mesenchymal stem cell; DE, definitive endoderm.