Table 2. Antibodies used for immunofluorescence and western blotting.

Marker (species)	Application (dilution)	Distributor (catalog number)
Primary antibodies
FoxA2 (rabbit)	IF (1:200)	Proteintech (22474-1-AP)
Sox17 (mouse)	IF (1:200)	R&D (MAB1924-SP)
AFP (rabbit)	IF (1:100)	Proteintech (14550-1-AP)
HNF4α (mouse)	IF (1:500)	Santa Cruz (sc-374229)
ALB (rabbit)	IF (1:200)	Proteintech (16475-1-AP)
A1AT (mouse)	IF (1:100)	Proteintech (66135-1-Ig)
ALB (rabbit)	WB (1:5,000)	Proteintech (16475-1-AP)
AFP (rabbit)	WB (1:1,000)	Santa Cruz (sc-8399)
A1AT (mouse)	WB (1:5,000)	Proteintech (66135-1-Ig)
HNF4α (rabbit)	WB (1:1,000)	ACTIVE MOTIF (61190)
NTCP (mouse)	WB (1:1,000)	Santa Cruz (Sc-518115)
FXR (mouse)	WB (1:1,000)	Santa Cruz (SC-25309)
GAPDH (rabbit)	WB (1:5,000)	Proteintech (10494-1-AP)
Secondary antibodies
Goat anti-rabbit IgG-HRP antibody	WB (1:10,000)	Boster (BA1051)
Goat anti- mouse IgG-HRP antibody	WB (1:10,000)	Boster (BA1054)
Goat anti-rabbit IgG secondary antibody	IF (1:100)	Boster (BA1032)
Goat anti-mouse IgG secondary antibody	IF (1:100)	Boster (BA1101)