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. 2021 Jul;9(13):1063. doi: 10.21037/atm-21-682

Figure 5.

Figure 5

PON1 regulates the expression of hepatic genes involved in HDL metabolism and inflammation. (A) Mouse PON1, Apoa1, Apoe, LDLR, LXRα, ABCA1, ABCG5, ABCG8, ACAT, IL-6 and TNFα mRNA levels were determined by qRT-PCR. mRNA levels of target genes were normalized to that of 18S, which was selected as an internal standard. n=4–5 per group. (B) Protein levels of APOA1, APOE, LDLR, LXRα, ABCA1, ABCG8, ACAT and p-NF-κB in mouse liver were detected by Western blot analysis. The expression level of each protein was normalized to that of GAPDH, which was selected as an internal standard. n=3–4 per group. The measurement data were presented as mean ± standard error of the mean. Data between the two groups were compared by unpaired Student’s t-test. *P<0.05, **P<0.01, versus Lv-GFP group. qRT-PCR, quantitative real-time polymerase chain reaction.