Figure 5.

Effects of BCCY-1 in specific cell-depletion mouse models. To deplete monocytes/macrophages, C57BL6/J mice were treated once with clodronate liposomes. Two days after liposome treatment, BCCY-1 (10 mg/kg/day, for 2 days) or saline was administered by i.p. injection. After 48 h administration, mice were infected with E.coli via i.p. injection. (A) The day of challenge was designated as day 0 (black arrow). Survival of mice (n=8-10) was measured and (B) bacterial CFU counts were determined in the peritoneal lavage at 24 h after infection. n=4-5, ns p > 0.05, *p < 0.05, **p < 0.01. (C) The bacterial CFU counts were determined in the peritoneal lavage fluid of the lymphopenia models at 24 h after infection. n=3-6, ns p > 0.05, *p < 0.05, **p < 0.01. (D) The bacterial CFU counts were determined in the peritoneal lavage of the neutropenic models at 24 h after infection. n=3-7, Cy: Cyclophosphamide, *p < 0.05, **p < 0.01.