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. 2021 Jul 22;12:707757. doi: 10.3389/fphys.2021.707757

FIGURE 4.

FIGURE 4

Relative mRNA abundance of heat shock protein (HSP)-27, HSP60, HSP70, and HSP90 in the blood of Cobb 500 male broiler chickens on day 42. Birds in all the groups were raised under the recommended conditions from day 0 to 28. Treatments include: (1) control (CTL): birds raised under thermoneutral condition (23°C) from day 29 to 42; (2) AHS: birds raised under thermoneutral condition (23°C) from day 29 to 42, but exposed to 2 h AHS (35°C) before sampling on day 42; (3) Pre-CHS: birds exposed to high ambient temperature (35°C) for 8 h/day (9:30 am–5:30 pm) from day 29 to 42, and sampled before starting the CHS; (4) Post-CHS: birds exposed to high ambient temperature (35°C) for 8 h/day (9:30 am–5:30 pm) from day 29 to 42, and sampled 2 h after starting the CHS; (5) pair-fed (PF): birds raised under thermoneutral condition (23°C) from day 29 to 42, but pair-fed to CHS group (these birds received each day 1.05 times the average feed intake recorded in the CHS group in the previous day). Values are represented as a n-fold difference relative to the calibrator (CTL). Results are given as means (n = 8) for each group. Error bars indicate standard errors. For each gene, bars with different letters are significantly different (P < 0.05).