FIGURE 2.

The general categorization of single cell epigenomic assays. (A) Methods that start by dissociating cells in a sample into a single-cell suspension followed by lysis of individual cells in physically separated compartments/reactions, reach high depth of information per cell, but are laborious and expensive and thus offer limited throughput. (B) Droplet based methods allow single-cell identification using barcoding without the need for physical compartmentalization of individual cells, and thus reduce cost and increase throughput. (C) Methods that use combinatorial barcoding on isolated nuclei offer many advantages including higher throughput, albeit in the expense of depth of information per individual cell.