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. 2021 Jul 21;93(30):10424–10434. doi: 10.1021/acs.analchem.1c02199

Figure 2.

Figure 2

Annotation of hCGα glycoforms. Deconvoluted mass spectra of hCGα obtained by HPLC-MS analysis (a) after desialylation or (b) without enzymatic treatment. Glycoforms are indicated by letters (A–D). Red asterisks indicate fucose variants. Numbers above the peaks refer to Neu5Ac residues. Peak lists with all possible glycoform assignments for untreated and desialylated hCGα are available in Supporting Information, Annotations hCGα BA059433. The instrument resolution setting was 140 000 at m/z 200.