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. Author manuscript; available in PMC: 2021 Aug 5.
Published in final edited form as: Nat Cell Biol. 2019 Sep 30;21(10):1234–1247. doi: 10.1038/s41556-019-0394-2

Figure 8. Phagosome resolution is mediated by contacts with the ER and by PtdIns4P.

Figure 8.

a) RAW macrophages were challenged with TAMRA-SRBC and phagosomes allowed to mature for 4.5 h: control macrophages were transfected with a control GFP vector (first column); ORP1L KO macrophages were transfected with either a GFP vector (second column); GFP-ΔORPSac1 (third column); or GFP-ORPSac1 (rightmost column). Magnifications of the green channel of the boxed area shown below. Scale bars = 10 µm. b) Assessment of phagosomal resolution by stages, defined as in Fig. 1; data are means ± SD of the number of determinations indicated below the brackets; significance assessed by two-tailed unpaired t tests. c) Hypothetical model of the regulation of phagolysosomal resolution by ORP1L-mediated contacts with the ER.