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. 2021 Jul 15;13(7):7641–7653.

Figure 4.

Figure 4

Maternal vitamin D supplementation before pregnancy promoted neural cell growth, while AMA inhibited neural cell growth in offspring’s hippocampus. A. Offspring in the 32 W+VD group and 8 W group had elevated hippocampal NEFH, NeuN, and MAP2 protein expression compared to offspring in 32 W group. Bar = 500 μm. B. Offspring in the 32 W+VD group and 8 W group had elevated hippocampal NEFH mRNA expression compared to offspring in 32 W group. C. No significant difference in NeuN mRNA expression was found between offspring hippocampi in the 32 W+VD group and 32 W group, or between the 8 W group and 32 W group. D. Offspring in the 32 W+VD group and 8 W group had elevated hippocampus MAP2 mRNA expression compared to offspring in 32 W group. All data are shown as mean ± SD, ns P>0.05, ***P<0.001.