Figure 5.

ERG activates CREs around CBF-1/RBP-Jκ pathway gene. A. Volcanic map of the Q-value corrected by -log10 FDR and the log2 fold change of acetylated of H3-lysine-27 during ERG knockout in NPA-87 cells (three independent copies in each group). B. -Log₁₀ FDR-corrected Q-value, which is used to calculate and enrich the regulation of CBF-1/RBP-Jκ signal pathway by CRE with tissues identified CORE or T2E-up CRE and significantly loss of activities after ERGs gene knockout. C-E. Expression of DLL1, JAG1 and HES1 mRNA in non-T2E or T2E sample from TCGA thyroid tumor cluster. F. After DMSO or DAPT treatment, the transmembrane cells stained with crystal violet migrated to NPA-87, TPC-1 and KTC-1 cells. G. Quantitative results of transwell migration with three independent repeats. *P < 0.05, two-tailed, one-sample t-test, Bonferroni-corrected; **P < 0.01, two-tailed t-test, Bonferroni-corrected.