Figure 2.

Knockdown of circRPPH1 restrained growth, metastasis and glycolysis of BC cells. A, B: Expression of circRPPH1 and its linear mRNA was detected by qRT-PCR after transfection with shRNAs targeting circRPPH1; C, D: CCK-8 and colony formation assay for the growth of BC cells; E, F: Transwell assays for evaluating BC cell migration and invasion (200×); G-I: The glycolysis of BC cells was assessed using commercial kits; J: Western blotting for determining PCNA, MMP9, and HK2 protein abundance; K, L: The tumor volume and weight were detected. Data are shown as mean ± standard deviation, **P<0.01, ***P<0.001.